NM from stock solutions

NM from stock solutions kdorfman Wed, 01/17/2018 - 14:35

DIW (pure deionized water) 780 ml

1M K2HPO4 (dibasic) 4 mL

1M KH2PO4 (monobasic) 16 mL

Difco Bacto Peptone (not Proteose Peptone) 3.2 g

Difco Bacto Agar 12.0 g

Mix by swirling (no need to dissolve). Autoclave 10 min. Cool to 45–50° in a water bath, and pour plates about ½ full.

To make plates (100 mm)
(for a final volume of mL of medium)
start with: mL water
add: mL 1M K2HPO4
add: mL 1M KH2PO4
add: g peptone (Difco Bacto Peptone, not proteose)
add: g agar
autoclave for: minutes