Mitotracker

Mitotracker kdorfman Wed, 02/21/2018 - 18:59

Mitotracker GREEN

Mitotracker GREEN kdorfman Thu, 10/24/2019 - 20:34

Thermo fisher M7514

Excitation 490 nm : Emission 516 nm

MW: 674

50 ug in vial.

Add 74 uL DMSO to make 1 mM stock.

working concentration =~ 25 - 200 nM

1 uL 1mM stock into 10 mL medium makes 100 nM treatment solution

Mitotracker RED

Mitotracker RED kdorfman Thu, 10/24/2019 - 20:34

Invitrogen M7512

https://www.thermofisher.com/order/catalog/product/M7513?SID=srch-srp-M…

Ex = 579 nm; Em = 599

50 ug per tube.

MW = 531

Add 100 uL DMSO to 50 ug in tube. Makes ~ 1 mM stock solution.

Use at 25 - 100 nM. (1 uL per 10 mL for 100 nM)

Dilute in medium. Treat with pre-warmed stain solution for 15 - 45 minutes. Replace staining solution with fresh (warm) medium.

To fix: use 3.7% formaldehyde (in medium); 37C for 15 minutes (??)