Poly-L-Lysine

Poly-L-Lysine kdorfman Thu, 04/26/2018 - 17:04

Non-specific attachment factor for cells; use it to coat coverslips.

Sigma P1524

  1. Add 50 ml of sterile tissue culture grade water to 5 mg of poly-lysine.
  2. Aseptically coat culture surface with 1 ml per 25 cm2 of solution. Rock gently to ensure even coating of the culture surface.
  3. After 5 minutes, remove solution by aspiration and thoroughly rinse surface with sterile tissue culture grade water.
  4. Allow to dry at least two hours before introducing cells and medium. If glassware or slides must be sterilized after coating with poly-lysine, γ-irradiation is recommended instead of autoclaving.

Can also buy poly-lysine coated slides: Fisher 6776215

sigmap1524.pdf