The orange runs fast; suitable for small DNA fragments

To make 10 mL from home made LD:

• 6 mL orange G (Fisher AAJ60562AC)
• 1 mL home made LD with bromophenol blue and xylene cyanacol
• 6 uL SYBR safe
• 1 mL glycerol
• 0.1 mL 1 M Tris pH 7.6
• water to 10 mL

Ethidium bromide

1 uL / 100 mL gel (or DNA sample, if adding it to the loading dye) (=1/100,000)

10 mg/mL
10 mL
17-ETBC1001 Krackeler $37.12

6X Home-made loading dye:

• 30% glycerol
• 0.3% Bromphenol blue
• 0.3% xylene cyanol

plus SYBR-Safe at 0.5 uL/1mL

1 uL loading dye stains 5 uL of DNA sample.

SYBR safe works at 1/10,000

need 5/10,000 uL for 5 uL of sample

so need 5/10,000 uL SYBR safe for each uL of loading dye.

= 5/10 uL for each mL

NOTE Fisher Tritrack loading dye (FERR1161) composition:

• 0.03% bromophenol blue
• 0.03% xylene cyanol
• 0.15% orange g
• 60% glycerol
• 10 mM Tris, pH 7.6
• 60 mM EDTA

One tenth as much dye, twice as much glycerol.

SYBR safe DNA gel stain

Fisher S33102

400 uL $72.62

Use 1 uL/10 mL gel or DNA sample (=1/10,000)

Safe-Green

(DNA stain + loading dye)

ABM G108-G
1 mL $65.00 ($30 + shipping)

Use at 1:5

Smart Glow loading dye

Krackeler 26510-E4500-LD
1mL $77.22

DNA stain + loading dye.

Add directly to DNA sample

Use at 1:5