LAMP1 (lysosomes) (mouse)
LAMP1 (lysosomes) (mouse) kdorfman Tue, 10/02/2018 - 16:22IgG
1 mg/mL
4C short term
Freeze 1 µL aliquots
dilute 1:100 - 1:750 in 0.1% BSA in PBS
rinse in PBS
Fix
- in 4% paraformaldehyde 15 min for endosomes
- in cold methanol for Golgi, 0.5% saponin 5 min
1% BSA in PBS 30 min
1o Ab stain (0.1% BSA) overnight at 4C
wash 30 min in PBS
2o Ab 1 h 37C
wash 30 min PBS
mount in DAPI