Naegleria 2019
Naegleria 2019 bcrcstaff Fri, 01/04/2019 - 16:48Naegleria 2019 schedule
Naegleria 2019 schedule bcrcstaff Fri, 01/04/2019 - 16:51| date | activities | supplies |
|---|---|---|
| 1/22 | discussion | none |
| 1/24 | Microscopy boot camp | slides for observation? |
| 1/29 | Cloning bootcamp | AgeI rSAP Ligase kit Qiagen PCR Purification 37C, 42 C heat block liquid LB LB amp (carb?) plates (3/pair), sterile glass beads Competent cells (DH5 alpha, just for cloning) |
| 1/31 | Naegleria 101 | HT115 (from Lil?), Lugol's loops 2 mM tris pH 7.6 30C shaking incubator sterile toothpicks liquid LB, carb or amp stock, coverslip bottom dishes sterile 10 mL culture tubes |
| 2/5 | Cloning bootcamp 2 transform dsRNA-expressing bacteria + controls |
miniprep restriction enzymes (which ones?) 1.2 % agarose gel (wide) TAE gel rig & viewer 20 carb/tet LB plates |
| 2/7 | start ON dsRNA bacteria cultures, prep Naegleria cultures | induction medium LB Amp 2 tubes of 5 mL/group |
| 2/11 | plate ON cultures | NM Amp/IPTG ( 0.5 - 1 mM) plates 2/ pair beads |
| 2/12 | grow bacteria. Plate lawns on induction medium | Naegleria & E. coli growth plates sterile beads |
| 2/14 | differentiate fed amoebae and controls | iodine (Lugols?) 2mM Tris culture tubes shaking incubator coverglass bottom dishes |
| 2/21 | presentations | none |