SC-AHLT low Ade mostly powder

High concentration agar is difficult to work with.

• Tends to boil over, so needs a lot of water in the autoclave pan
• Hard to get into solution - frequently needs re-autoclaving
• So viscous that bubbles don't rise to the surface

So

• mix the ingredients that can't be autoclaved in as little water as possible
• Only use high concentration stock solutions for micro-ingredients

Per Liter:

dry ingredient	g
SC-AHLT	1.64
YNB	1.7
Ammonium sulfate	5
glucose	20

stock solutions	mL
10X Adenine hemi sulfate	2.5
50X Tryptophan	20
100X Histidine HCl	10

plus water to 150 mL

filter sterilize

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Autoclave

• 20 g agar
• 850 mL water

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Mix and pour

Mark with one green and one blue line.