VTM
VTM kdorfman Fri, 04/03/2020 - 14:42Viral Transport Medium
Equipment and materials needed
- Sterile Hood
- Waterbath at 56C to heat-inactivate FBS
- Sterile serological pipets
- Sterile 15 mL tubes
- Filter sterilization
Reagents
- HBSS 1X with calcium and magnesium, without phenol red
- FBS, heat inactivated
- Gentamicin sulfate (50 mg/mL)
- Amphotericin B (Fungizone) (250 ug/mL)
- Sheep blood agar plate (or equivalent) for quality control