HU (Hydroxyurea)
HU (Hydroxyurea) kdorfman Wed, 01/06/2021 - 18:29| ingredient | working conc | MW | stock |
|---|---|---|---|
| HU | 0.2 M | 76 | Koshand lab 2M in H2O |
Hydroxyurea: (Sigma H8267-1g)
- To make 50 mL YPD with 0.2M HU
vi = (0.2M * 50 mL)/2M = 5 mL
100µL 2M stock per mL medium
Buy 1 g, make 6.6 mL 2M stock
- MW = 76, so 1 g = 1/76 mol = 0.0132 mol
- vol = (1000 mL/2 mol) * 0.0132 mol = 6.6 mL
See yeast recipes for QBoC https://wahoo.cns.umass.edu/node/554/