Lab 5.1 prep

Lab 5.1 prep kdorfman Wed, 08/25/2021 - 20:48

11/18 & 11/30 2021

11/18 Thursday

Group 1: Botros et al.

  • Zone of inhibition
  • E. coli Addgene 20972 from molecular biology experiment
  • LB Amp 25 mL
  • alcohol (ethanol or isopropanol?)
  • disinfectant??
  • 20 LB amp plates for zone of inhibition
  • sterile discs
  • sterile forceps

Group 2 (Vengalil et al.):

Group 3 (Long, et al.):

  • 40 LB plates (no antibiotic)
  • Replica plate materials
  • Kid's hand sanitizer
  • room swipe access (need names and Spire IDs)

Group 4 ( Lee, et al.):

  • E. coli GFP culture in LB Kan
  • LB Kan ~25 mL
  • LB-glu Kan ~15 mL
  • 96 well black plate, shared with group 5
  • rewrite script for only the rows they use

Group 5 (Creto, et al.)

  • E coli GFP culture in LB Kan
  • LB Kan ~25 mL
  • LB-glu Kan ~15 mL
  • LB-lac Kan ~15 mL
  • LB-gal Kan ~15 mL
  • 96 well black plate, shared with group 4
  • rewrite script for only the rows they use

11/30 TUESDAY

McKnight, Carson, Matfes, Berke

  • change pH of LB
  • GFP expression
  • plate reader

Bowen, Howall, Abraham, Wald

  • fertilizer - N-based vs P-based.
  • Nitrogen vs phosphorus fertilizer
  • LB,
  • sterile tubes,
  • plates
  • Culture (non-GFP?)

Boyd, Ebian, Melusen, Manning

  • pH again

Cloutier, Dawiczyk, Tan, Joyce

  • mouthwash
  • plate reader
  • culture (non-GFP?)
  • Liquid LB

Alexander, Ferriera, Beinstein, Birnbach

  • comparison of various substances at killing E. coli
  • LB broth and plates
  • E coli culture (not GFP?)

Crotty, Galligan, Horrigan, Jansen

  • NaCl
  • KCl
  • plate reader overnight
  • agar plates