3.5 Salk genotypes

Identifying genotypes of Salk-line plants: Analysis

Goal for this lab: Determine the genotypes of Salk line plants to identify homozygous mutant individuals. Run PCR reactions from 3.4 on gels

Students will have 28 + samples, so they need two gels.

Materials:

  • 2 2% agarose gels per pair
  • 1× TAE running buffer (no EtBr)
  • Loading dye (2µL/lane)
  • Fermentas MassRulerTM DNA ladder, low range (5µL/gel)