Cells Wed 11/14

For Wed, 11/14/12

Names cell type # dish/coverslip reagents
Jonathan & Laura LL parentals 5 slips fixative, mounting medium
Mike & Theresa 3t3 4 dishes transferrin, Fe-HBS, HBS
Mike & Mike LL alpha-GFP, myo-RFP 4 dishes blebbistatin, non-CO2 medium
Cassie & Marc LL parentals 4 dishes nuc-blue, HBS, Fluo-4, non-CO2
Dave & Tyler LL alpha-GFP 2 dishes, 2 slips Fix, mitotracker, Rhodamine123, non-CO2, PBS
Mike & Katherine LL parentals 3 dishes ceramide, non-CO2, PBS
Ray & Poya LL alpha-GFP 2 dishes non- CO2

Plating:

  • LL parental: 5 coverslips, 7 dishes
  • LL alphas: 4 dishes, 2 coverslips
  • LL alpha, myo: 4 dishes
  • 3t3: 4 dishes