DEB

DNA Extraction Buffer for Gene & Genome

100 mM NaCl, 50 mM Tris, pH8, 25 mM EDTA, 1% SDS

Make from stock solutions

To make mL DNA Extraction Buffer
add: mL 5M NaCl
add: mL 1M Tris, pH8
add: mL 500mM EDTA
add: mL 20% SDS
add: uL BMe AT THE LAST MINUTE!

ingredient cf ci 100 200 mL
NaCl 100 mM 5000 mM 2 4 mL
Tris pH 8 50 mM 1000 mM 5 10 mL
EDTA 25 mM 500 mM 5 10 mL
SDS 1 % 20 % 5 10 mL
BME 10 mM 12564 mM 79.5 159 µL (at last minute!)