LPGM

Lilly pollen growth medium

205 mM (7%) sucrose (See Sugars.)

1.6 mM H3BO3

0.1 mM CaCl2

15 mM MES, pH 5.7 with KOH

To make mL LPGM
add: mL 150 mM MES
add: uL 10 mM CaCl2
add: uL 160 mM Borate

Stock solutions:

150 mM MES

10 mM CaCl2

160 mM Borate

? 35 % Sucrose (filter sterilze)