Spermidine

Spermidine:

  • neutralizes and stabilizes the negative charge on the DNA phosphate backbone
  • displaces other ions from DNA
  • stimulates restriction enzyme reactions
  • increases specificity and reproducibility of Taq-mediated PCR

Used in Gene & Genome

1 M stock

1 g spermidine (entire contents of bottle) in 6.9 mL water

MW = 145.25

Filter sterilize (0.22 µm filter, 10 mL syringe)

Freeze 200 uL aliquots

20 mM student solutions

1 g spermidine (entire contents of bottle) in 6.9 mL water

MW = 145.25 Make 20 mM solution for students by adding 800 uL to the 200 uL of 1M in the tube, then aliquot 10 uL for each student.

To make µL 20mM Spermidine
add: µL 1M spermidine
to: µL water