Thawing

  • Label flask with cell line, passage number, date
  • Put 10 mL medium in flask, set in incubator
  • Retrieve frozen vial from LN2 dewar, thaw in incubator or water bath.
  • Put ~1 mL thawed cells into a single flask
  • 24 hours later, replace medium (5 mL)
  • Split as needed