Vasopressin

1 mM stock in water
(NO CALCIUM, so stock can be used for Ca-free solutions in Lab 7.2)

1 mg in bottle, MW = 1084
Add 0.92 mL to make 1mM stock

1 µL aliquots in freezer

1 µM dilution to make student solutions

1 µL 1 mM in 999 µL water

(NO CALCIUM, so stock can be used for Ca-free solutions in Lab 7.2)

10 nM (= 1.084 µg/mL) for students

NOTE: Rather weak response in 2011. Try doubling the concentration?

1 part 1µM dilution to 99 parts HBS

Make enough for Labs 7.1 and 7.2: Lab 7.1: 0.750 mL per coverslip x 4 coverslips x 7 groups = 21 mL Lab 7.2: 0.75 mL per coverslip x 2 coverslips x 7 groups = 10.5 mL

Make 40 mL: 400 µL 1µM Vasopressin + 39.6 mL HBS

Make 24 aliquots of 1.6 mL (16 for Lab 7.1, 8 for Lab 7.2