PBS

Submitted by kdorfman on Tue, 06/16/2009 - 17:52

Make 1X PBS from 10X

100 mL 10X + 900 mL H2O

10X PBS from Fisher

Fisher BP399-4 $51.07 as of 05/2020 ($34.45 January 2018!)

https://punchout.fishersci.com/shop/products/phosphate-buffered-saline-…

10X PBS from stock solutions

from Sigma ready-made 1X:

M ingredient
0.01 Na-K Phosphate
0.138 NaCl
0.0027 KCl
To make L 10X PBS
add: mL 5M NaCl
add: mL 1M KCl
add: mL 1M KH2PO4
add: mL 1M Na2HPO4.7 H2O (MW = 268.07)

pH to 7.3 with NaOH

sterilize

10X PBS from dry ingredients (Carrie's recipe)

To make L 10X PBS
add: g NaCl (MW = 58.44)
add: g KCl (MW = 74.55)
add: g KH2PO4 (MW = 136.09)
add: g Na2HPO4.7 H2O

pH to 7.3 with NaOH

sterilize