SC-HLT Ade-free Concentrate

SC-HLT Ade-free Concentrate kdorfman Wed, 02/02/2022 - 19:18

Concentrate to make SC-L High Ade or Low Ade agar

Make this concentrate, then add adenine hemi-sulfate.

for low-Ade, add 5 mg Ade per L of concentrate

for high-Ade, add 1.6 g Ade per L of concentrate

to make high-Ade from low-Ade concentrate, add 1.595g Ade per L, or 0.1595g per 100 mL

Then filter sterilize. For every L of medium, combine 20g agar autoclaved in 875 mL water with 125 mL warmed up concentrate.

add 125 mL concentrate to autoclaved 875 mL water + 20 g agar

to make enough concentrate for:	Liter(s) SC-L high Ade or SC-L low Ade agar medium
start with	mL water
add:	g YNB
and:	g ammonium sulfate
and:	g SC -HLT
autoclave for:	minutes (to get it into solution)
while it's still hot, add:	g glucose
and:	g tryptophane
and:	mL his 100X concentrate.
Bring to a final volume of:	mL 8X SC-HLT Ade-free. Add the appropriate amount of Ade and filter sterilize