ConA dishes for 383H

Materials:

• con A aliquots

• cleaned (but not necessarily sterile coverslip dishes (check size so they'll fit into the adaptors for the microscope stage)

• 10 mL S2 medium in a conical tube

Protocol

• Students put conA in non-sterile dishes

• When they split their cultures, they put 1 mL of cells into a microfuge tube.

• In class, they put cells onto the treated coverslip, then add medium after they are stuck.