Con A coverslips

Submitted by kdorfman on Sun, 05/24/2020 - 15:38

Requirements:   * Acid washed coverslips stored in 100% ethanol.

  • ConA 0.5 mg/mL. 

  • Large petri dishes to store the coverslips.    


  • Flame the acid-washed coverslips and cool for 10 sec.    
    (Make 9-14 coverslips at a time to save time.)

  • Place the coverslips on top of the large petri dish.     

  • Thaw the conA from the freezer and dilute to 0.5 mg/mL 

  • apply around 400uL to the first two coverslips 

  • Wait for 10 sec. 

  • Tilt the coverslips and suck out the excess ConA 

  • Repeat the same for all the coverslips. 

  • Cover the petri dish with another lid of petri dish and allow a small opening to dry.

  • If possible,use a vacuum pump  to dry it faster.   

The process usually takes 15 minutes and drying takes half an hour. 


  * In a petri dish, place the coverslips on top of a clean kimwipe with the conA surface facing up.

  • Cover with the lid. 10 min before needed, UV sterilize them.

  • Alternatively, UV sterilize all of them after drying and open the dishes only in aseptic conditions or inside hood.