Requirements: * Acid washed coverslips stored in 100% ethanol.
ConA 0.5 mg/mL.
Large petri dishes to store the coverslips.
Flame the acid-washed coverslips and cool for 10 sec.
(Make 9-14 coverslips at a time to save time.)
Place the coverslips on top of the large petri dish.
Thaw the conA from the freezer and dilute to 0.5 mg/mL
apply around 400uL to the first two coverslips
Wait for 10 sec.
Tilt the coverslips and suck out the excess ConA
Repeat the same for all the coverslips.
Cover the petri dish with another lid of petri dish and allow a small opening to dry.
If possible,use a vacuum pump to dry it faster.
The process usually takes 15 minutes and drying takes half an hour.
* In a petri dish, place the coverslips on top of a clean kimwipe with the conA surface facing up.
Cover with the lid. 10 min before needed, UV sterilize them.
Alternatively, UV sterilize all of them after drying and open the dishes only in aseptic conditions or inside hood.