Con A coverslips

Requirements:   * Acid washed coverslips stored in 100% ethanol.

• ConA 0.5 mg/mL. 

• Large petri dishes to store the coverslips.    

  Procedure:

• Flame the acid-washed coverslips and cool for 10 sec.    
  (Make 9-14 coverslips at a time to save time.)

• Place the coverslips on top of the large petri dish.     

• Thaw the conA from the freezer and dilute to 0.5 mg/mL 

• apply around 400uL to the first two coverslips 

• Wait for 10 sec. 

• Tilt the coverslips and suck out the excess ConA 

• Repeat the same for all the coverslips. 

• Cover the petri dish with another lid of petri dish and allow a small opening to dry.

• If possible,use a vacuum pump  to dry it faster.   

The process usually takes 15 minutes and drying takes half an hour. 

Storage:

  * In a petri dish, place the coverslips on top of a clean kimwipe with the conA surface facing up.

• Cover with the lid. 10 min before needed, UV sterilize them.

• Alternatively, UV sterilize all of them after drying and open the dishes only in aseptic conditions or inside hood.