(Purchased 2022 for NAP lab Bio 388)
Store powder at -20
Make 0.05M stock solution: 0.068 g in 5 mL DMSO. vortex
|Synonyms||β-Estradiol, Estradiol, 17β-Oestradiol, E2|
|Stability||≥2 years at -20°C|
|Supplied as||A crystalline solid|
|UV/Vis||λmax: 281 nm|
|solubility||2.5 mg/mL in ethanol
20 mg/mL in DMSO
0.2 mg/mL in a 1:4 solution of DMSO:PBS (pH 7).
Dissolve in DMSO first!
Use within a day
Estrogens direct the development of the female genotype in embryogenesis and at puberty. 17β-Estradiol is the major estrogen secreted by the premenopausal ovary. It is synthesized from testosterone primarily in the ovarian granulosa cells and placenta, but small amounts can be produced in the adrenal gland.1,2 Plasma 17β-estradiol levels increase gradually between days 1-7 of the menstrual cycle followed by a sharp increase to a peak value of about 300 pg/ml on day 12, just prior to ovulation