Taxol (20 µM final)
Fisher 109710 Paclitaxel.
Sigma T7402 1mg
MW = 853.91
- Stabilizes the microtubule polymer
- Protects microtulues from disassembly.
- Prevents metaphase spindle configuration
Blocks progression of mitosis and prolonged activation of the mitotic checkpoint triggers apoptosis or reversion to the G-phase of the cell cycle without cell division.
soluble in DMSO, not water.
Add 0.117 mL DMSO to make a 0.01M (=10 mM) stock solution from the 1 mg powder
Aliquot 10 µL 10 mM (if it precipitates, add 10 uL DMSO and vortex, then add 480 uL medium or buffer)
- add 490 µL to make 200µM
- dilute into medium from there (100 uL Taxol/mL medium)
- Printer-friendly version
- Log in to post comments