Nocodazole

Submitted by kdorfman on Mon, 04/23/2018 - 17:51

Prevents polymerization of microtubules. Prevents cells from entering metaphase.

*Acros 358240100, 10 mg

*Soluble in DMSO

*Dilute stock in medium to treat cells.

*Make a 10 mg/mL (=33mM) stock: Add 1 mL DMSO to 10 mg in bottle.

  • Aliquot 3 µL 33 mM per tube.

*Working concentration range is usually 100nM - 30 µM.

  • add 97 µL to tube to make 100 µL of 1 mM

  • 33 µL 1mM + 10 mL medium = 3.3 µM