Restriction Enzymes

Submitted by kdorfman on Fri, 08/26/2022 - 15:50

Updated fall 2022

Diluent B

Enzyme CutSmart NEB1 NEB2 NEB3 notes
Aat2 100 10 50 50
Acc651 25 10 75 100
Aci1 100 10 25 100
AflII 100 50 100 10
Age1 75 100 75 25 tinybit
AlwN1 100 10 100 50
Apal1 100 100 100 10
Asc1 100 10 10 10
Ava1 100 10 100 25
Ava2 100 50 75 10
BamHI-HF 100 100 50 10
BciVI 100 100 25 10 almost empty
Bgl2 10 10 10 100
BmgB1 10 10 10 100
BmsAI 100 50 100 100 tiny amount
BsaI 100 5 75 100
BspD1 100 25 75 50
BspE1 10 10 10 100 from 2007
BsrB1 100 50 100 100
BsrD1
BsrG1 25 25 100 100
Bsu361
Cla1 100 10 50 50
Dra1 100 75 75 50
Eag1-HF 10 10 25 100
Ear1 100 50 10 10
EcoR1-HF 50 25 100 50
EcoRV-HF 100 25 100 100
HaeIII 100 50 100 25
Hind3 50 25 100 50
Hind3HF 100 10 100 10
HinP1I 100 100 100 100
Hlu1-HF
Hpa1 100 10 75 25 x2
Hpa2 100 100 50 10
Kan1HF x2
Kas1 100 50 100 50
KpnI-HF 100 100 25 10
Mbo1 100 75 100 100
Mlu1 25 10 50 100
Mnl1
Msp1 100 75 100 50
Nae1 100 25 25 10
Nco1 100 100 100 100
Nda1
Nde1 100 75 100 100
Nhe1-HF 100 100 25 10
Psha1
Pst1 50 75 75 100
Pvu1 10 10 25 100 x2
Pvu2 100 50 100 100
Rsa1 100 25 50 10
Sac1 100 100 50 10
Sal1HF 10 10 10 100
Sara1
Sau3A1 100 100 50 10 almost none
Sca1 NR NR NR 100
SexA1 100 100 75 50
Sma1 100 10 10 10
Spe1 (NEB) 100 75 100 25
Spe1GQ (Promega)
Sph1 100 100 100 50
SSpI 50 50 100 50
Stu1
Sty1 10 10 25 100 lots
Sty1-HF 100 25 100 25
Xba1 100 10 100 75
Xcm1 100 10 100 25
Xho1 100 75 100 100
Xma1

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