CMBL 2022

Submitted by kdorfman on Mon, 01/31/2022 - 12:33

reagents

  • 1M DTT (25 ml; filter sterilized and stored at -20)
  • 50% PEG 3350
  • 1M Lithium Acetate pH 7.5
  • Qiagen miniprep kits
  • Yeast beta-galactosidase assay kit (Thermo Fisher #75768--3 kits)

plates

  • SC-leu low adenine plates (350 plates; we will use 50 on 2/4, 50 on 2/11, and 200 on 2/21)
  • SC-leu high adenine plates (75 plates; we will use 50 on 2/11 and 25 on 2/25)
  • old plates of any kind (25-30...these are just for practicing spotting cultures and maintaining sterility)