Non-specific attachment factor for cells; use it to coat coverslips.
- Add 50 ml of sterile tissue culture grade water to 5 mg of poly-lysine.
- Aseptically coat culture surface with 1 ml per 25 cm2 of solution. Rock gently to ensure even coating of the culture surface.
- After 5 minutes, remove solution by aspiration and thoroughly rinse surface with sterile tissue culture grade water.
- Allow to dry at least two hours before introducing cells and medium. If glassware or slides must be sterilized after coating with poly-lysine, γ-irradiation is recommended instead of autoclaving.
Can also buy poly-lysine coated slides: Fisher 6776215