Biological Activity for DAPT (Info from Tocris 2634)
Purchased from Fisher: AAJ65864MA
Mfr: Thermo Scientific Chemicals J65864MA
M. Wt: 432.46 Formula: C23H26F2N2O4 Store at +4°C Light sensitive
Solubility: 43.24 mg/mL (100 mM) in DMSO
DAPT is a γ-secretase inhibitor. DAPT reduces Aβ40 and Aβ42 levels in human primary neuronal cultures (IC50 values are 115 and 200 nM for total Aβ and Aβ42 respectively) and in brain extract, cerebrospinal fluid and plasma in vivo. DAPT has no effect on APPα and APPβ levels. DAPT blocks Notch signaling in hybrid human-mouse fetal thymus organ culture (FTOC) and causes ESCs to commit to neuronal differentiation. DAPT can be used in a small molecule cocktail to derive cortical neurons from hPSCs and to maintain hepatocytes in culture. DAPT also promotes the formation of cone photoreceptors in retinal organoids.