Primers

Fisher Custom Oligos

• Primers come lyophilized.

• Tube label says how many nmol in the tube (usually ~100-500)

• Multiply nmol x 10 = μL of sterile water to add to make 100 μM stock. Fisher primer calculator

• This is the only stock!

• Put it away safely on instructor shelf in freezer.

• Make a working stock for students (give them all of it):

• 12.5 μM (12.5 μL of the 100 μM stock + 87.5 μL water).
  

(If using the repeater pipet for the water, 85 µL water + 12.14 µL 100 µM stock)