Yeast Lab week of 10/2/23

(See also pollination)

YED plates: 4/pair

Mutagenesis

See notes here

• Sterile technique
• Spray 70% ethanol
• Serial dilution (5 tubes)
• Sterile water
• glass beads
• Wild type stock plate (HA0 or HB0) (1/table)
• YED plates 3/pair
  • control (most dilute)
  • 7 sec UV exposure (next most dilute)
  • 10 sec UV exposure (next most dilute)
• UV transilluminators
• face shields

Come in next day and move plates to fridge

Look for spores

• check YeKAc plate from previous lab; make a wet mount and look for spores
• Microscopes
• slides
• coverslips
• water
• pick from YeKAc plate to YED plate (1/pair) to observe for color next lab

Observe class "pre-spore" YED plate from previous week