YED

Submitted by kdorfman on Mon, 02/08/2016 - 19:22

Yeast Extract Dextrose

For Bio 284

Remember to autoclave an appropriate sized graduated cylinder to measure the glucose solution in the sterile hood.

NOTE: 900 mL may boil over in a 1 L bottle in the autoclave. Try:

  • Autoclave:
    • YE + agar in 800 mL
    • 100 mL H2O
    • mix in BSC
  • Add 100 mL sterile 20% glucose
  • mix and pour
To make YED agar plates
at mm plate diameter
pour mL per plate
start with: mL water
add: g yeast extract, stir till dissolved
add: g agar, leave stir bar in
autoclave for: minutes
add asceptically mL filter sterilized 20% glucose
for a final volume of mL

Mark with a single red line.