- 0.15 g YNB
- 0.52 g ammonium sulfate
- 2.0 g agar
- 82 mL H2O
- 8.0 mL 1 mg/mL adenine
- 10.0 mL 20% glucose (final conc = 2g/100 mL)
Minimal Vitamin Medium plus Adenine
Label plates with "+ ADE"
Make sure it's a plus sign!
Autoclave an appropriately sized graduated cylinder to measure the glucose and adenine solutions.
Mark with two blue lines
*Yeast Nitrogen Base without amino acids and ammonium sulfate