Yeast Extract Adenine Dextrose Medium
Ade mutants grow well on this without turning red. Better for keeping the parental strains white longer.
1 gram Yeast Extract 2 grams anhydrous dextrose (glucose) 2 grams Agar 8 mL adenine stock solution (1 mg/mL)
Adenine Stock Solution
400 mg adenine in 400 ml water (1 mg/ml) Store at room temperature. Use 2 ml stock solution for 100 ml of medium; reduce the water added to the medium by 2 ml.
Remember to autoclave an appropriate sized graduated cylinder to measure the glucose solution in the sterile hood.