YEKAC

Submitted by kdorfman on Mon, 02/08/2016 - 19:29

Sporulation Medium for Bio 284

If you have concentrated potassium acetate solution, then:

  • 20.4 mL 5M KOAc per liter
  • 12.75 mL 8M KOAc per liter Fisher AAJ63372AE
To make YEKAC agar plates
at mm plate diameter
at KOAc Molarity
pour mL per plate
for a final volume of: mL water
start with: mL water
add: mL KOAc, stir till dissolved
add: g yeast extract, stir till dissolved
add: g agar, leave stir bar in
autoclave for: minutes

Mark with a single black line.

If you are using potassium acetate powder, then:

To make YEKAC agar plates
at mm plate diameter
pour mL per plate
start with: mL water
add: g potassium acetate, stir till dissolved
add: g yeast extract, stir till dissolved
add: g agar, leave stir bar in
autoclave for: minutes