Submitted by kdorfman on Mon, 02/08/2016 - 15:04

Minimal Vitamin Medium

Autoclave an appropriately sized graduated cylinder to measure the glucose solution.

To make MV agar plates
pour mm plate diameter
pour mL per plate
start with: mL water
add: g YNB*, stir till dissolved
add: g ammonium sulfate, stir till dissolved
add: g agar, leave stir bar in
autoclave for: minutes
add asceptically mL filter sterilized 20% glucose
for a final volume of mL

*Difco Yeast Nitrogen Base w/o Amino Acids and w/o Ammonium Sulfate

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