PCR Calcs

PCR Calcs kdorfman Mon, 04/01/2013 - 20:56

From Takara Ex Taq recipe

For	PCR reactions
at	µL per reaction
add	µL 10X Ex Taq buffer
add	µL dNTP 2.5 mM
add	µL forward primer 12.5 µM
add	µL reverse primer 12.5 µM
add	µL template DNA (~250 ng/µL)
add	µL water to final volume
add	µL ExTaq 1U/µL
for a final volume of	µL

TaKaRa ExTaq manual