Plant growth media
Plant growth media kdorfman Thu, 05/23/2013 - 16:0210 g agar per L
1/2 MS low Iron
1/2 MS low Iron kdorfman Tue, 02/15/2022 - 15:001/2 Murashige & Skoog, 1µM iron medium
MS 10x micronutrients is 100 µM FeSO4, so MS complete is 10 µM, 1/2 MS is 5µM
FeSO4 stock is 10 mM, which is 10,000x 1 µM
LPGM
LPGM kdorfman Wed, 08/31/2016 - 20:24MS high salt
MS high salt kdorfman Mon, 02/03/2014 - 15:33150 too high - try 100mM for F 2015
150 mM NaCl
10 g agar/L
Make regular MS, then add 0.03 mL 5M NaCl per mL medium
100 mM NaCl
0.02 mL 5M NaCl per mL medium
MS iron-free
MS iron-free kdorfman Thu, 05/23/2013 - 17:46Murashige & Skoog 1µM iron medium
MS 10x micronutrients is 100 µM FeSO4, so MS complete is 10 µM
FeSO4 stock is 10 mM, which is 10,000x 1 µM
10 g agar/L
MS low iron
MS low iron kdorfman Thu, 05/23/2013 - 17:40Murashige & Skoog 1µM iron medium
MS 10x micronutrients is 100 µM FeSO4, so MS complete is 10 µM
FeSO4 stock is 10 mM, which is 10,000x 1 µM
MS medium
MS medium kdorfman Thu, 05/23/2013 - 16:021/2 MS
1/2 MS kdorfman Wed, 12/22/2021 - 20:25*Add the other salt mixtures to the water to prevent precipitation
**pH to 5.7 with 1M KOH (initial pH = ~3.66) (needs~21 drops or ~720 µL/L)
Autoclave with stir bar in flask or bottle
Stir until cool enough to handle
Pour 30 mL per plate (use the deep ones)
Pour 50 mL per square plate (20 plates per liter)
[1]: Sigma M 0654 Murashige and Skoog basal salt macronutrient solution (Krackeler 45-M0654-1L-EA) ~$26
[2]: Sigma M 0529 Murashige and Skoog basal salt micronutrient solution (Krackeler 45-M0529-1L-EA) ~$26
1X MS
1X MS kdorfman Wed, 12/22/2021 - 20:37*Add the other salt mixtures to the water to prevent precipitation
**pH to 5.7 with 1M KOH (initial pH = ~3.66) (needs~21 drops or ~720 µL/L)
Autoclave with stir bar in flask or bottle
Stir until cool enough to handle
Pour 30 mL per plate (use the deep ones)
[1]: Sigma M 0654 Murashige and Skoog basal salt macronutrient solution (Krackeler 45-M0654-1L-EA) ~$26
[2]: Sigma M 0529 Murashige and Skoog basal salt micronutrient solution (Krackeler 45-M0529-1L-EA) ~$26