E2 medium (Zebrafish)
E2 medium (Zebrafish) kdorfman Sun, 01/13/2019 - 21:48Use the attached file while this set of pages is under construction
E2 Embryo Medium final Working Solution: 0.5X E2
To make 20 L, start with 19 L RO water, and add
ml | component |
---|---|
100 | 100X E2A |
20 | 500X E2B |
20 | 500X E2C |
10 | 0.1% MeBlue (optional) |
Then bring to final volume
0.1% Methylene Blue
0.1% Methylene Blue kdorfman Thu, 03/19/2020 - 14:531 gram of methylene blue per liter of RO water.
Shake well to dissolve.
Store at room temp.
0.5X E2A
0.5X E2A kdorfman Thu, 03/19/2020 - 14:54E2A (100x)
E2A (100x) kdorfman Thu, 03/19/2020 - 14:51FOLLOW THE RECIPE IN THE ATTACHED FILE (omitting methylene blue). THIS PAGE IS NOT DONE YET
100X E2:
1.5 M NaCl
50 mM KCl
100 mM MgSO4
15 mM KH2PO4
5 mM Na2HPO4