5-mC (rabbit)

5-methylcytosine (D3S2Z)

Rabbit mAb

Binds to methylated cytosine

28692S Cell Signaling Technology

From the manufacturer: "Methylation of DNA at cytosine residues is a heritable, epigenetic modification that is critical for proper regulation of gene expression, genomic imprinting, and mammalian development (1,2). 5-methylcytosine is a repressive epigenetic mark established de novo by two enzymes, DNMT3a and DNMT3b, and is maintained by DNMT1

Dilute 1:1600

Before Antibody treatment, treat fixed cells with 4N HCl for 15 minutes to denature DNA.

Neutralize with 2 washes with sodium borate pH 9, then PBS. Then start the PBS-Tw-Az rinses before incubating with the antibody.

Store at -20