G&GA solutions

G&GA solutions kdorfman Fri, 01/13/2012 - 17:55

Make solutions (Check supplies first)

See stock solution recipes

Tris pH8 1M 25 mL
Tris pH7.5 1M 25 mL
EDTA 500mM 200 mL
NaOAc 3M 10 mL
KOAc 5M 100 mL
SDS 20mM 25 mL
NaCl 5M 25 mL
DEB 200 mL
T10E1 100 mL
T10E5 10 mL
TAE 50x 1 L
MassRuler™ DNA Ladder, High Range Fermentas #SM0393 (not 2018?)
MassRuler™ DNA Ladder, Low Range Fermentas #SM0383 (not 2018?)
MassRuler™ DNA Ladder Mix, SM0403 FERSM0403 (print 2 copies to put on the gel docs)
6X loading dye:
- 30% glycerol
- 0.3% Bromphenol blue
- 0.3% xylene cyanol

Aliquot:

Solution	vol		aliquots/pair	total aliquots	for Labs
T10E1	1	mL	5	50	1.1, 1.2, 2.4, 3.3
T10E5	0.75	mL	2	20	1.1
EtOH 95%	1.5	mL	2.2	22	1.1, 5.4
EtOH 70%	7.5	mL	1.2	12	1.1
isopropyl	7.5	mL	1.2	12	1.1
NaOAc	100	µL	1.2	12	1.1
KOAc	5	mL	1.2	12	1.1
DEB	10	mL	1.2	12	1.1
Tris pH 7.5 10 mM	1.5	mL	3	30	1.2, 5.4
loading dye	50	µL	1.5	15	1.2, 2.4, 2.5, 3.4, 5.4, 5.6
HMW std	15	µL	1.2	12	1.2
dNTP 2.5 mM	150	µL	1.5	15
sterile H2O	1	mL	6	60	2.4, 2.5, 3.3, 5.5
LMW std	50	µL	1.5	15	2.4, 2.5, 3.4, 5.4, 5.6

fermentas_calcs.xls

solutions calcs

solutions calcs kdorfman Fri, 07/12/2013 - 20:59

Reagent	vol/aliquot	aliquots/pr	tot aliquots	total vol
For	pairs
T10E1				mL
T10E5				mL
EtOH 95%				mL
Isopropyl				mL
NaOAc				mL
KOAc				mL
Loading dye				mL
Mass ruler mix				mL
dNTP 2.5 mM				mL
Sterile water				mL
Tris pH 7.5 10 mM				mL

solutions_calc_template.txt