PCR Calcs

PCR Calcs kdorfman Mon, 04/01/2013 - 20:56

From Takara Ex Taq recipe

For PCR reactions
at µL per reaction
addµL 10X Ex Taq buffer
addµL dNTP 2.5 mM
addµL forward primer 12.5 µM
addµL reverse primer 12.5 µM
addµL template DNA (~250 ng/µL)
addµL water to final volume
addµL ExTaq 1U/µL
for a final volume ofµL