Mussel projects

Mussel projects kdorfman Wed, 02/08/2017 - 22:21
  • Make 2 L Instant Ocean, aliquot
  • Change pH with NaOH, HCl, see how long it lasts
  • hot block or digital hot plate
  • order epinephrine (bought 1 g from Acros, should try solution: Fisher S25126 L-Adrenaline Solution, 0.01% = 0.01g/100 mL = 0.1 mg/mL, 100 mL $6.37) solubility = 0.18 mg/mL
  • Make 10X acetylcholine, serotonin
  • Check ATP supply
  • find thermometers
  • vasopressin
  • nicotine (10^-3, 10^-4, 10^-5, 10^-6 in instant ocean)
  • Lactic acid
musselmuscleexperiments.pdf

F2017 Mussels

F2017 Mussels kdorfman Mon, 09/18/2017 - 18:53

Projects

  • 2L Instant Ocean

  • CaCl

  • Adrenaline Cl 1:10,000, 100 mL (0.1mg/mL)

    • Fisher S25126
    • L-Epinephrine CAS# 51-43-4
    • 183.207 g/mol
    • 180 mg/L (at 20 °C). Very slightly soluble in alcohol. Readily soluble in aqueous solutions of mineral acids, NaOH, and KOH. Insoluble in aqueous solutions of ammonia and of the alkali carbonates. Insoluble in chloroform, ether, acetone, oils.

  • Creatine Phosphate

    • Fisher AC 337340010 1 g $55.95
    • MW 321.14
    • H2O: 0.1 g/mL, clear, colorless
    • 32 mg/1 mL =~100mM

  • Nicotine

    • Fisher AC181420050
    • 99%

  • Serotonin

  • acetylcholine