Mirus kdorfman Fri, 12/06/2019 - 14:36

general summary

100 uL Mirus reagent
2 ug DNA(up to 20 uL DNA solution)
1 - 5 million cells/mL Lonza Knowledge Center


  • Warm medium in flask and coverslip-bottom dish
  • Mix DNA and Mirus reagent; warm up
  • Trypsinize cells:
    • remove medium
    • rinse with warm PBS
    • add 0.5 mL trypsin
    • incubate till all cells are in suspension (at least 3 min)
    • add 1 mL COLD medium
    • mix well by pipetting up and down
  • transfer all to sterile 2 mL tube
  • spin 500 x g 3 minutes
  • resuspend cells in Mirus/DNA solution
  • put all into sterile cuvette, close
  • Put cuvette into Nucleofector
  • Choose program1
  • Press the button
  • Use special dropper to transfer one drop to the coverslip dish and the rest to the waiting flask.

Mirus nucleofection kit

  1. Cell type Program #
    B16 P-031
    3t3 U-030
    HCT116 D-032
    HeLa I-013
    LLCPk-1 X-001


Cell Density

Cell Density kdorfman Wed, 09/29/2021 - 16:47
See estimating cell numbers
If cells are % confluent
in a sq cm flask
there are roughly million cells in the flask