Estimating cell numbers

Submitted by kdorfman on Tue, 08/22/2023 - 17:23

From Useful Numbers for Cell culture

(See also counting cells)

Container Surface area (cm2) Seeding density* Cells at confluency mL growth medium
35 mm dish 8.8 0.3 x 106 1.2 x 106 2
60 mm dish 21.5 0.8 x 106 3.2 x 106 5
100 mm dish 56.7 2.2 x 106 8.8 x 106 12
150 mm dish 145 5.0 x 106 20.0 x 106 30
6-well plate 9.6 0.3 x 106 1.2 x 106 1 to 3
12-well plate (~20 mm) 3.5 0.1 x 106 0.5 x 106 1 to 2
24-well plate (~15 mm) 1.9 0.05 x 106 0.24 x 106 0.5 to 1.0
48-well plate 1.1 0.03 x 106 0.12 x 106 0.2 to 0.4
96-well plate 0.32 0.01 106 0.04 x 106 0.1 to 0.2
T-12.5 flask 12.5 0.35 x 106 1.4 x 106 1.5-2.5
T-25 flask 25 0.7 x 106 2.8 x 106 3–5
T-75 flask 75 2.1 x 106 8.4 x 106 8–15
T-175 flask 175 4.9 x 106 23.3 x 106 35–53
T-225 flask 225 6.3 x 106 30 x 106 45–68
  • Seeding density is given for each culture vessel type as follows:
    • Dishes and Flasks: Cells per vessel;
    • Culture plates: Cells per well
Estimating Cell Numbers