• Put medium into dishes
    • MatTek dishes for observations of live cells
    • coverslip in a 35 mm dish for fixation and mounting
  • Let dishes equilibrate in incubator
  • Treat cells in flask with trypsin as for splitting
  • Stop trypsinization with cold medium
  • Add 1 - 4 drops of medium + cells to each dish, depending on cell density
  • Check density of first dish, adjust volume for the remaining dishes
  • Check after 24 hours.