2018 cell schedule

Submitted by kdorfman on Wed, 12/20/2017 - 18:48
Lab date number cell line live or fixed in stained with notes
1&2 1/23 10 LL parental fixed in paraglut phalloidin learn about scope
3&4 1/30 10 LL parental fixed in paraglut tubulin, phalloidin, DAPI NA
5 2/6 10 LL parental (dense) fixed in paraglut tubulin, phalloidin, DAPI photobleaching
6 2/8 none Image J, photoshop
7 2/13 30 LL parental fixed in paraglut unstained immunofluorescence
10 3t3 fixed in paraglut unstained
10 LL parental live on coverslip to fix and stain
8 2/15 Lab practical
9 2/20 3 LL parental fixed in MeOH gamma tubulin 1:100 (mouse) (C) organelle ID lab
3 LL parental fixed in MeOH Hec1 1:200 (mouse) (didn't do)
3 LL parental fixed in MeOH LAP2 1:200 (mouse) (B)
3 LL parental formaldehyde only Alpha actinin (mouse IgM) 1:200 (D)
3 LL parental formaldehyde only Golgi 58K 1:100 (mouse) (A)
3 LL parental doesn't matter secondary Ab only
9 2/22 10 LL a-tub GFP/RFP myosin live in dish imaging live cells
18 LL live in dish for mitotracker or ceramide
10 LL H2B m cherry live in dish transfected unknowns
10 2/27 GFP E. coli, motile E. coli, Volvox Carolina 152655, Tetrahymena Carolina 131620, Dictyostelium Carolina 55996, Paramecium caudatum Carolina 131554, Chaos (Pelomyxa) carolinensis Carolina 131324
11 3/1 10 3t3 live in dish motility
10 LL parental live in dish
10 melanoma live in dish
10 LL GFP-actin live in dish
12 3/6 9 3t3 live in dish Motility II
12 LL GFP-tub
3 LL GFP actin
5 B16
13 3/8 Posters
14 3/20 26 3t3 live on coverslip Endocytosis I
3/22 18 3T3 live on coverslip Endocytosis II
10 LL parental live on coverslip
18 LL a-tub-GFP live on coverslip
13 3/27 28 3t3 live in dish (dense) Signalling 1
3/29 28 3t3 live in dish (dense) Signalling 2
14 4/3 10 LL-a-tub-GFP live in dish mitosis, cytokinesis
18 LL myo tub live in dish
15 4/5 10 LL- parental live on coverslip for fixation
10 LL GFP-tub (or tub & myo) live in dish for treatment, observation