Cell passaging

Students split a flask of B16s into a new flask and a coverslip-bottom dish for viewing next class

• 1 flask of B16-30 (wild type) per pair (at least 50% confluent)

• 1 flask, 1 coverslip-bottom dish per student

• 1 50 mL tube of DMEM per pair

• 1 15 mL tube of PBS per pair

• 1 0.5 mL aliquot of trypsin per pair

• 5 mL serologicals

• sterile transfer pipettes

• 70% ethanol spray bottles

• lots of gloves

• handout