1.1

Submitted by kdorfman on Mon, 08/19/2013 - 17:14

Materials

  • one balance per table (borrow from Bill Schmitt)
  • weighboats
  • non-sterile tips
  • Mats
  • Gloves
  • small glass culture tubes (~15/pair)
  • rack for tubes

  • beaker for water

  • blue flashlights & yellow filters

Computers and Plate Readers

  • Check program Repeatability (Abs 485 nm; layout A1-B12)
  • Wahoo folders must be named Tues01, Tues02, ...Tues12 and Thur01, Thur02 ... Thur12, even though the user names in the Optima program are Tue01 and Thu01, etc.
  • Import program to groups
  • Check network connections (in “my computer” connect the Z drive to Wahoo as kdorfman, kd@ISB)

10mM NaOH

MW = ~40

10mM = 0.01M = 0.4g/L

To make M NaOH/
with g NaOH pellets
use L H2O

1 mM Fluorescein

in 10 mM NaOH

It fades with time, so check the concentration of the old stocks.

MW = 332

1mM = 0.332g/L = 0.033g/100 mL

A = Ecl

Molar extinction coefficient of fluorescein at 485nm = 50358/mol.cm

so Absorbance 10^-5M = 0.50358

(1/100 of a 1mM solution =10^-5M)