Strain RP437 - wildtype for chemotaxis
At least 2 days before lab:
Streak all chemotaxis strains on LB agar.
Night before lab
Pick a single colony from the RP437 plate, inoculate into ~5 mL minimal medium + HMLTT; shake at 30C
Morning of lab
Inoculate ~200µL into ~45 mL minimal medium + HMLTT
At class time, OD should be ~0.02
Don't let them reach stationary phase!