Paraffin Embedding (Rats)

Submitted by kdorfman on Fri, 05/30/2014 - 18:24

Do all of the following at room temperature.

1.) Fix in Bouin’s (Bouin's Fixative Solution (Fischer) Cat No.: 112016) overnight in tubes.

This step should be kept short and should not include agitation. I have noticed that the intestine and liver could both benefit from shorter fixation times.

2.) Place tissue in cassettes and rinse for 2 hours in running water, then place into 70% ETOH (they may be stored here for up to several months).

There is a large jar with a lid to perform all of the following steps in.

3.) Cellosolve (Ethylene Glycol Monobutyl Ether (Fischer) Cat No.: E179-4) I – 1-2 hours, or longer.

4.) Cellosolve II – 1-2 hours, or longer.

5.) Cellosolve III – 1-2 hours, or longer.

6.) Cellosolve IV – Overnight.

7.) Toluene I – 1 hour.

8.) Toluene II – 1 hour.

9.) Paraffin I – 1 hour.

10.) Paraffin II – 1 hour.

11.) Paraffin III – 1 hour under vacuum (vacuum not necessary).

12.) Embed